Round two submitting samples to AGRF. First attempt directly from agar and stored in >95% ethanol for a month or two did not yield DNA. This time I've grown cultures in liquid (PD broth) for two weeks and submitted to AGRF. We should have some feedback for this approach next week... I'm already optimistic. We've submitted seven haploid cultures of P. subaeruginosa and three of P. cubensis. Plenty of P. cubensis around southeast Queensland at the moment. We're starting to exhaust our sampling locally and it will be time to move further afield. The aim of this part of the project is to determine whether gold tops are introduced, and if yes, how many times. We suspect it is an introduction and will sequence about 50 genomes from 10 or more locations (separated by ≥ 100 kms). If we observe high genetic diversity, we will need to re-think our sampling.
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Leela Rizal
12/13/2021 12:37:38 pm
Wish I could collect magic mushrooms, describe, culture, identify them, characterize genetically, investigate their mineral contents, mineral absorbing potential from the soils, microbial activities, and domesticate them on the best substrate for secondary metabolites. I have domesticated some edible wild mushrooms in Thailand. To me, Mushrooms are Majestic, Magically, Medicinal and Magnificent.
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Designer Shrooms @ Funky Fungus on 1st July 2023
I started a gig at Funky Fungus as Chief Scientific Officer to make designer shrooms Our research on Psilocybe
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