Thank you to AGRF for the high quality genomic data that we have since assembled. Each sample had 19–27 million reads (about 6–8 Gb of data). I was worried that I didn't sequence Psilocybe, however, our rDNA contigs are a perfect match for P. subaeruginosa and P. cubensis (pictured above). Phew. (The resolution of the tree is atrocious, if you're desperate to see the taxon selection, there is a pdf here). My next worry was that I had contaminated haploid cultures with dikaryotic cultures. What a relief, everything that is meant to be haploid, was haploid. The below figure shows that the one dikaryon (essentially a diploid) we sequenced was a much worse assembly than our haploids. I'll use this information to only sequence haploids in the future. Next step is to improve these assemblies with some long reads from PacBio. Stay tuned for updates on what we pull out from these genomes in the next few weeks... with a decent annotation, we may already be able to make a case that P. cyanescens is con-specific with P. subaeruginosa, depending on their similarity. Genome assemblies for Psilocybe cubensis (first three on the left) and P. subaeruginosa. The circle size is proportional to genome size, and number of contigs is centred in circles. The far right assembly was a dikaryotic culture of P. subaeruginosa, all other assemblies were haploid. We want the number in the circle to be as small as possible to reflect the number of chromosomes.
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Designer Shrooms @ Funky Fungus on 1st July 2023
I started a gig at Funky Fungus as Chief Scientific Officer to make designer shrooms Our research on Psilocybe
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